Neymar has not played since suffering a rib injury against Montpellier on February 1, forcing him to miss league wins over Nantes and Lyon, as well as the midweek Coupe de France quarter-final triumph over Dijon.
With Borussia Dortmund coming up in the Champions League next week, PSG boss Thomas Tuchel has opted against taking any risks with the Brazil international.
Mbappe played the entire game in the 6-1 thrashing of Dijon on Wednesday, including scoring his side's second goal just before half-time, but will not be involved at all against Amiens.
"We won't take any risks, obviously, but at the same time we'll put out a strong team," Tuchel had said at his pre-match press conference on Friday, prior to naming his squad.
Abdou Diallo, Presnel Kimpembe, Layvin Kurzawa and Thomas Meunier are also absentees for the game against relegation-threatened opponents, along with Pablo Sarabia.
However, Marco Verratti and Marquinhos are included in a 19-man squad as the reigning champions aim to move 15 points clear of nearest rivals Marseille, who are away at Lille on Sunday.