Barcelona and Paris Saint-Germain will do battle in a repeat of their memorable Champions League last-16 tie from three years ago, while holders Bayern Munich were drawn against Lazio in Nyon on Monday.
LaLiga giants Barca became the first team in the competition's history to overturn a 4-0 first-leg deficit when they famously defeated PSG 6-1 in the return tie at the same stage in the 2016-17 season.
Neymar scored twice and assisted Sergi Roberto's last-gasp winner on that occasion but his status to face his former club with PSG may be in doubt after the Brazil superstar sustained a serious-looking ankle injury in Sunday's 1-0 loss at Lyon.
Barca also defeated PSG in the quarter-finals en route to treble glory in 2014-15 and in the last eight in the 2012-13 campaign.
PSG were runners-up in a competition delayed by the coronavirus pandemic last term, and their conquerors Bayern were paired against Serie A side Lazio.