Benjamin Mendy has left the France squad after being diagnosed with a knee injury, while Alexandre Lacazette has also been replaced.
The French Football Federation (FFF) confirmed on Monday that Arsenal striker Lacazette has withdrawn after a consultation between national team doctor Franck Le Gall and Gunners medical staff.
The 27-year-old had only been officially added to the France squad on Monday, after Manchester United's Anthony Martial pulled out with a groin problem.
The FFF did not specify the injury to Lacazette, who has been replaced in the squad by Alassane Plea.
However, they did confirm that Manchester City full-back Mendy has suffered "a sprained left knee and a meniscal injury" and will return to his club for treatment.
Lyon defender Ferland Mendy has replaced his namesake in Didier Deschamps' set-up.
France face Netherlands in the Nations League on Friday before a friendly match with Uruguay four days later.