The dramatic development comes in the wake of Japan's world number two declaring she would not fulfil press conference duties during the tournament at Roland Garros.
She cited mental health concerns for reaching that decision, and Osaka now says she plans a break from tennis, which may mean she does not play at Wimbledon.
In her withdrawal announcement, Osaka said she has suffered "long bouts of depression" since winning the US Open in 2018.
Grand slam chiefs surprisingly escalated the situation on Sunday by declaring that repeated violations of their code of conduct could see Osaka thrown out of the event.
Now Osaka has taken the matter into her own hands, a day after winning her first-round match.
She wrote on Twitter: "Hey everyone, this isn't a situation I ever imagined or intended when I posted a few days ago. I think now the best thing for the tournament, the other players and my well-being is that I withdraw so that everyone can get back to focusing on the tennis going on in Paris.
"I never wanted to be a distraction and I accept that my timing was not ideal and my message could have been clearer.
"More importantly I would never trivialize mental health or use the term lightly.
"The truth is that I have suffered long bouts of depression since the US Open in 2018 and I have had a really hard time coping with that."