The Barcelona player sustained the injury in a collision with Breel Embolo in Saturday's 1-1 draw in Switzerland, a result which allowed Germany to replace Spain at the top of the group. Luis Enrique's side must win Tuesday's match in Seville to qualify for the Nations League finals next October. France became the first team to reach the four-team finals at the weekend. Busquets is also expected to be ruled out of Barcelona's La Liga clash against Atletico Madrid in the Spanish capital on Saturday.