The defender has been a key fixture in Carlo Ancelotti's side since the Italian took over, but he suffered a muscle injury in a 2-1 friendly win over Egypt on Sunday (AEST).

Wesley was forced off in the 17th minute and left the stadium on crutches, having been seen crying on the bench after he was helped off the pitch by the medical team.

Ancelotti conceded after the game that Brazil was worried about the serious-looking injury, with further tests confirming the Roma full-back will not be available for the tournament.

"The CBF regrets the injury. Wesley is a beloved player within the group and will always be considered part of this team that is seeking its sixth world championship," a statement from the Brazil team read.

In a social media post, Wesley said: "Not every battle is won on the field.

"I have to interrupt a dream because of an injury. It hurts not being able to wear the Brazilian national team shirt right now, but those who know my story know that giving up was never an option.

"I have come a long way. I've been through hardships, faced challenges, and had to overcome many obstacles just to get here. Nothing has been given to me. Every achievement is built with hard work, faith, sacrifice and a lot of resilience.

"So, I face this moment in the same way I faced everyone else: with my head held high, with trust in God, and the certainty that I will come back even stronger.

"The fall is part of the journey. Getting up has always been my greatest strength."

As such, Ancelotti has called up a midfielder as his replacement, with Ederson, who has reportedly agreed a deal to move to Manchester United, set to join the squad on Monday.

Ederson has previously made three appearances for Brazil, but none in 2026 so far, with his most recent cap coming in March 2025.

The Selecao begins its 2026 FIFA World Cup campaign against Morocco on 14 June (AEST), with Haiti and Scotland also to follow in Group C.