A group of 36 players will meet in Brighton next Monday for a five-day camp before heading to continue their preparations for the tournament at Pennyhill Park.
Orlando Bailey, Alfie Barbeary, Ollie Chessum, Tommy Freeman, Ollie Hassell-Collins and Luke Northmore have been called up for the first time.
England faces Scotland in its first Six Nations match at Murrayfield on 6 February AEDT.
Red Rose head coach Jones said: "Selecting this squad has been a difficult task, we've got plenty of good young players coming through and some of our more experienced are rediscovering their best form.
"We think this 36 for the first training week reflects a good balance of that experience and up-and-coming talent.
"In Brighton we’ll focus on getting the fundamentals of our game in play right and developing the cohesion of the team.
"The Six Nations is going to be the most competitive we've ever seen. All the countries performed well in the autumn, so we need to be at our best and improve with every game."
Ben Youngs is poised to become England's most-capped player during the tournament, as the scrum-half is just two appearances away from Jason Leonard's record tally of 114.
Forwards: Alfie Barbeary, Jamie Blamire, Ollie Chessum, Luke Cowan-Dickie, Tom Curry, Alex Dombrandt, Charlie Ewels, Ellis Genge, Jamie George, Joe Heyes, Jonny Hill, Maro Itoje, Courtney Lawes, Lewis Ludlam, Joe Marler, Bevan Rodd, Sam Simmonds, Kyle Sinckler, Will Stuart.
Backs: Mark Atkinson, Orlando Bailey, Owen Farrell, Tommy Freeman, George Furbank, Ollie Hassell-Collins, Max Malins, Joe Marchant, Jonny May, Luke Northmore, Jack Nowell, Raffi Quirke, Harry Randall, Henry Slade, Marcus Smith, Freddie Steward, Ben Youngs.