The Manchester City-linked striker put a temporary end to speculation regarding his future by declaring that he is "100 per cent focused" on Spurs.
England captain Kane was not included in Tottenham's squad for their first two games of the campaign, but he returned as a second-half substitute in the win at Wolves.
He has been named in Spurs' squad for the Europa Conference League play-off tie with Pacos, which the north London outfit trails 1-0 from last week's first leg.
Tottenham boss Nuno Espirito Santo is happy to be able to move on from the transfer saga, which has somewhat overshadowed his side's positive start to the new Premier League season.
"It's great news, fantastic news for everybody," Nuno said when asked about Kane committing his future to Spurs.
"I think since Harry returned to us, he's been working hard. This is what we value. His attitude in training has been great.
"It's now finished – Harry's going to be with us. He worked today and is an option for tomorrow. Like I said, fantastic news for everybody.
"We are all very happy. He is one of the best players in the world. We are all very happy, delighted to have one more option to the season ahead of us."
Kane has scored 221 goals in 337 appearances for Tottenham in all competitions and is second only to Jimmy Graves (266) in the list of the club's all-time top scorers.
The 28 year-old's 37 goal involvements last season were his most across a league campaign to date, while his 14 assists doubled a previous high of seven in 2016-2017.
Despite Kane admitting in May that he was at a "crossroads" in his career, Nuno has no doubt over the prolific striker's motivation to continue playing for Spurs.
"I think it's up to us and everybody, the player himself, to get this motivation. There shouldn't be any kind of doubts to pay for Spurs," Nuno said.
"This is an amazing club, a good team, and we we need everybody involved during this process. It's good hat this issue is over and we can proceed as normal."
Tottenham made 11 changes to its starting line-up for last week's trip to Portugal.
Spurs must now turn things around when Pacos visit Tottenham Hotspur Stadium, with Nuno expected to name a stronger side this time around.
When further probed on whether Kane is in line to start, Nuno said: "Let's see, we still have to decide. He trained today. It was a fantastic training session.
"But we still have to decide the starting team for tomorrow. There are a lot of things to understand. This week is different, we are all together.
"That allows us to make different decisions, different circumstances. It's a must win game, with the tie to be decided tomorrow."