The trio join Denis Shapovalov, Felix Auger-Aliassime and Diego Schwartzman for the team event which runs from September 24-26 at TD Garden in Boston.

Laver Cup newcomer Opelka rose to a career-high world number 23 ranking en route to his first ATP Masters 1000 final in Toronto and defeated world number three Stefanos Tsitsipas, who will play for Bjorn Borg's Team Europe.

Isner, who has featured for Team World since the inaugural event in 2017, reached the semi-finals in Toronto and claimed his 16th ATP Tour title in Atlanta at the start of August.

He described the Laver Cup as "a highlight of my year", adding: "To be on a team with guys we're normally competing against is so different and so much fun. We come together so well as a group, the chemistry is awesome and it's such a great environment to be part of."

Australian firebrand Kyrgios is a striking inclusion in Team World's roster, while Team Europe will be without their big three: Roger Federer, Rafael Nadal and Novak Djokovic.

Federer and Dominic Thiem were expected to take part in this year's event, though both were forced to withdraw with injuries.

However, Borg's men still boast six of the world's top 11. World number two Daniil Medvedev leads the line-up, with Tsitsipas and Tokyo Olympics gold medallist Alexander Zverev for company.

Casper Ruud, who collected a 14th win in his last 15 completed matches on tour when he beat Opelka, will feature, while Andrey Rublev and Wimbledon finalist Matteo Berrettini complete the six-man team.

Team Europe has landed the title in each of the three editions of the tournament so far, with Prague, Chicago and Geneva having served as hosts.