Ivo Karlovic – one of only two tennis stars taller than the giant Isner – had set the benchmark with 13,728 across his career, the most since aces were first tracked in 1991.
But Isner has chipped away at that total over the course of this week at the All England Club.
The American's 54 aces in five sets against Enzo Couacaud in the first round made him the first player to pass 50 in a grand slam match since he had last done so versus Steven Johnson at the 2020 US Open.
And there were a further 36 in an epic second-round win over Andy Murray, leaving Isner just four short of matching Karlovic and five away from passing him.
Against Jannik Sinner, in Friday's third round, Isner raced past his target, tallying 12 aces in the first set alone.
It is a record Isner is likely to own for some time, with 43-year-old Karlovic absent from the ATP Tour this year and playing only six main-draw matches in 2021.
Roger Federer is third on the list, way back on 11,478.