South Africa head coach Jacques Nienaber announced his team on Tuesday, and the 23-man group features 21 players who helped South Africa to their global triumph two years ago in Japan.
Handre Pollard, who will act as vice-captain, will earn his 50th cap as the three-Test series gets under way in Cape Town.
Ox Nche and Makazole Mapimpi, with Kolisi, were the last to join the camp in Cape Town on Monday but all have proved their fitness following the self-isolation period in Johannesburg.
Nienaber's men breezed past Georgia 40-9 in a warm-up Test two weeks ago but the scheduled second match was cancelled due to coronavirus outbreaks in both camps, meaning Saturday will be just the Springboks' second Test since the World Cup success.
The Springboks opt for changes on the wing, where Mapimpi and Cheslin Kolbe will start, and in the centres where Damian de Allende resumes his partnership with Lukhanyo Am.
South Africa make two further changes to their replacements, with Lood de Jager of Sale Sharks and Rynhardt Elstadt of Toulouse replacing Marvin Orie and Jasper Wiese.
Warren Gatland is expected to reveal his 23-man Lions selection on Wednesday as the tourists prepare for the highly anticipated series opener.
South Africa: Willie le Roux, Cheslin Kolbe, Lukhanyo Am, Damian de Allende, Makazole Mapimpi, Handre Pollard, Faf de Klerk; Ox Nche, Bongi Mbonambi, Trevor Nyakane, Eben Etzebeth, Franco Mostert, Siya Kolisi (captain), Pieter-Steph du Toit, Kwagga Smith.
Replacements: Malcolm Marx, Steven Kitshoff, Frans Malherbe, Lood de Jager, Rynhardt Elstadt, Herschel Jantjies, Elton Jantjies, Damian Willemse.