A number of members of the Baa-Baas squad left the team hotel without permission or informing organisers about their whereabouts.
The players in question were separated from the rest of the group in the bubble upon their return in order to protect its integrity.
Those individuals will not be permitted to remain with the squad and the game will only go ahead if players with an appropriate COVID-19 testing history can be brought in to replace them.
The Rugby Football Union (RFU) confirmed on Thursday it is reviewing if the Quilter Cup match can be staged and an update on the fixture will be provided at 3pm local time on Friday.
Details of who is in or out of the Barbarians squad will only be disclosed if it is confirmed the game will be played.
The RFU arranged the game to give Eddie Jones' side a warm-up fixture ahead of their decisive final Six Nations encounter with Italy in Rome a week on Sunday.