The 24 year-old striker sustained the muscle problem in the first half of PSG's 3-1 Ligue 1 victory at Montpellier and is set to miss around three weeks of action.
The French champion released a statement to reveal Mbappe's estimated recovery time, which if accurate would mean he will miss league games against Toulouse, Monaco and Lille, as well as the Coupe de France clash with Marseille and its Champions League first leg at home to Bayern, which takes place on 14 February.
"After examinations, Kylian Mbappe suffered from a lesion of the left thigh at the level of the femoral biceps. His recovery time is estimated at three weeks," PSG said.
Mbappe – who has 25 goals in 26 appearances for PSG this season – had a game to forget on Wednesday, having also had a penalty saved, including a retake before somehow also lifting the rebound over the bar, and then coming off with his injury in the 21st minute.
The club also confirmed that Sergio Ramos will undergo "further examinations" after the veteran Spanish centre-back also came off against Montpellier having appeared to suffer a head injury.
