The Manchester United defender collided with Spain's Dani Carvajal early in the second half and was unable to continue after receiving treatment on the field for almost seven minutes.
Thank you for all the love and support I am doing fine and am in the best hands. I’m a fighter so I will be back soon! ❤💪🏼— Luke Shaw (@LukeShaw23) September 8, 2018
Despite suffering what looked like a serious injury, he was treated by doctors at Wembley rather than being rushed to hospital and his message in the aftermath of the game helped to soothe supporters' fears.
In a message on Twitter, he wrote: "Thank you for all the love and support I am doing fine and am in the best hands.
"I'm a fighter so I will be back soon."
The game against Spain saw Shaw, who has been blighted by injuries throughout his career, make his first start for England in three years and his first Three Lions appearance since March 2017.
Shaw received support from a number of other players in the game, also, including Spain.
Hope to see you soon on the pitch,Luke👏🏻👏🏻👏🏻— César Azpilicueta (@CesarAzpi) September 8, 2018
What a ball by @LukeShaw23, really hope your alright pal 💪🏾— Marcus Rashford (@MarcusRashford) September 8, 2018