Dortmund did not elaborate on the reasons why Aubameyang was banned, but reports suggests that the club were unhappy with him partying while on international duty and filming an unauthorised video with a freestyle footballer on its premises.
Bosz's men lost 2-1 to promoted Stuttgart in the Bundesliga on Sunday without the striker, as extra pressure was mounted on the coach.
Dortmund goes into Wednesday's match with little hope of qualifying for the knockout phase, having accumulated just two points from four matches, leaving them five adrift of second-placed Real Madrid.
A win for Zinedine Zidane's men against APOEL on Wednesday will confirm Dortmund's group stage exit, but Bosz will at least feel his side's chances for the visit of Spurs are boosted by Aubameyang's return.
"Aubameyang plays tomorrow [Tuesday]," Bosz said.
"I talked with him and also the team. He has trained with the team today [Monday], that's why I can say that [he will play] so adamantly."
Dortmund has been in poor form of late, winning just two of its last nine matches across all competitions, reportedly leaving Bosz in danger of getting sacked.
Bosz understands the pressure and disgruntlement among fans, however, acknowledging that it is just a part of life with a big club.
"I understand the unrest," he said. "That's how it has to be if you work for a big club like Dortmund - you have to win. I feel that people understand the phase which we are in."
Midfielder Julian Weigl insists that any displeasure from the supporters has not translated to friction among the players, though.
"Of course we talk a lot about our situation," the youngster said. "We have intense discussions. Everyone has different views and different solutions to our problems.
"There are also guys who are close to each other, but there was no exaggerated friction or dispute."