The 27 year-old got through 90 minutes in a Premier League 2 match, almost a year after tearing meniscus cartilage in his knee while on international duty with Argentina.
The news will be warmly welcomed by Sam Allardyce after the Toffees manager last week confirmed knee surgery would sideline Eliaquim Mangala for the remainder of the season.
Everton has not kept a clean sheet in 10 Premier League matches and Funes Mori is eager to help arrest a poor run of form.
"It felt good to play back in Goodison, to feel the pitch, the stands - I really miss it, so I'm very happy," he said. "I've been training good these past weeks. Even though I wasn't playing for the first team, I've been playing for the Under-23s to get the rhythm, get the tempo. I'm 100 per cent fit to play and I'm ready for Saturday."
Funes Mori could still come into contention for the World Cup in Russia if he impresses Argentina boss Jorge Sampaoli.
The defender debuted for the Albiceleste in 2016 and has gone on to win 19 caps.