France star Mbappe completed 90 minutes in last week's first leg, which City won 2-1 thanks to second-half goals from Kevin De Bruyne and Riyad Mahrez, but sustained a calf problem.
The Argentinian striker will do so again at the Etihad Stadium, flanked by Neymar and Angel Di Maria in an all-South American forward line.
Former Manchester United man Ander Herrera comes into the PSG midfield in place of Idrissa Gueye, who is suspended following his red-card challenge on Ilkay Gundogan in the initial encounter, while Pochettino has plumped for Abdou Diallo ahead of Mitchel Bakker at left-back.
Pep Guardiola's side also sees alterations in those two positions, with Oleksandr Zinchenko given the nod over Joao Cancelo at left-back and club captain Fernandinho a surprise selection ahead of usual first choice Rodri at the base of the midfield.
Here we go! 💙— Manchester City (@ManCity) May 4, 2021
XI | Ederson, Walker, Dias, Stones, Zinchenko, Fernandinho (C), Gundogan, Bernardo, Mahrez, Foden, De Bruyne
SUBS | Steffen, Carson, Ake, Sterling, Jesus, Aguero, Laporte, Rodrigo, Torres, Mendy, Cancelo
🔷 #ManCity | https://t.co/axa0klD5re pic.twitter.com/yLNkXasjbf
City have never reached a Champions League final, but on the previous 47 instances of an English side winning the first leg of a knockout tie away from home in Europe's top competition, they have progressed on each occasion.