Giovanni Reyna, Chris Richards and Konrad de la Fuente have been called up by the United States for the first time.
Head coach Gregg Berhalter has announced the 24-man roster for November's upcoming friendly games against Wales and Panama.
The USMNT will face Wales in Swansea on November, before traveling to Wiener Neustadt, Austria to face Los Canaleros on November 16.
Borussia Dortmund midfielder Reyna, Bayern Munich defender Richards and Barcelona youngster De la Fuente were among 10 uncapped players in the group, which has an average age of just 21 years and 300 days, and only six players with more than 17 caps.
Berhalter said: "We are excited about getting this group back together. I'm proud of the way the players have handled the challenging times – they really stuck together.
"We have built a solid foundation, and now we get a chance to continue our work together ahead of what is going to be a critical 12 months for our team."
Reyna has two goals and four assists – three of which came in a 4-0 win over Freiburg last month – in nine appearances in all competitions for Dortmund this season. He will be hoping to become the 12th player to earn a USA cap before turning 18 years old.
Richards, 18, has featured three times for Bayern in all competitions and set up Robert Lewandowski's second goal in the 4-3 triumph over Hertha Berlin on October 4.
De la Fuente is yet to make his first senior appearance for Barcelona but the 19-year-old has been named on the bench for three LaLiga matches this season.
The USA will take on Wales in Swansea on November 12 before travelling to Austria for a meeting with Panama.
England youth international Yunus Musah, 17, has also been handed a chance to take his first steps in the senior arena by Berhalter.
USMNT ROSTER BY POSITION (CLUB; CAPS/GOALS):
GOALKEEPERS (3): Ethan Horvath (Club Brugge/BEL; 4/0), Chituru Odunze (Leicester City/ENG; 0/0), Zack Steffen (Manchester City/ENG; 17/0)
DEFENDERS (7): John Brooks (Wolfsburg/GER; 38/3), Reggie Cannon (Boavista/POR; 10/0), Sergiño Dest (Barcelona/ESP; 3/0), Matt Miazga (Anderlecht/BEL; 18/1), Tim Ream (Fulham/ENG; 40/1), Chris Richards (Bayern Munich/GER; 0/0), Antonee Robinson (Fulham/ENG; 7/0)
MIDFIELDERS (6): Tyler Adams (RB Leipzig/GER; 10/1), Johnny Cardoso (Internacional/BRA; 0/0), Richard Ledezma (PSV Eindhoven/NED; 0/0), Weston McKennie (Juventus/ITA; 19/6), Yunus Musah (Valencia/ESP; 0/0), Owen Otasowie (Wolverhampton/ENG; 0/0)
FORWARDS (8): Konrad de la Fuente (Barcelona/ESP; 0/0), Nicholas Gioacchini (Caen/FRA; 0/0), Christian Pulisic (Chelsea/ENG; 34/14), Uly Llanez (Heerenveen/NED; 1/1), Gio Reyna (Borussia Dortmund/GER; 0/0), Josh Sargent (Werder Bremen/GER; 12/5), Sebastian Soto (Telstar/NED; 0/0), Tim Weah (Lille/FRA; 8/1)