Alba went off at half-time in the 0-0 draw at Camp Nou on Tuesday, with Sergi Roberto replacing the full-back for the second half.
LATEST NEWS❗️Tests performed have shown that @JordiAlba has had a relapse of the injury to his left biceps femoris. He is out and his recovery will determine his availability. pic.twitter.com/LxTeSPGbTb— FC Barcelona (@FCBarcelona) November 5, 2019
Spain international Alba was sidelined for several weeks with a hamstring injury earlier on in the campaign, and Barca have revealed the 30-year-old has suffered a reoccurrence of the same problem.
Barca, who confirmed the news on their official Twitter channel, offered no timescale on Alba's return.
"Tests performed have shown that Alba has had a relapse of the injury to his left biceps femoris [hamstring]. He is out and his recovery will determine his availability," the club's tweet read.
Barca's draw left Ernesto Valverde's side without a win in their last two matches, and they look set to be without Alba for Saturday's LaLiga match against struggling Celta Vigo.