Alessandro Bastoni, Gian Marco Ferrari and Mattia Zaccagni have been added to Italy's squad for their upcoming triple-header of fixtures.
Centre-backs Bastoni and Ferrari will provide cover for Angelo Ogbonna and Alessio Romagnoli, who both picked up injuries for their club sides over the weekend.
Bastoni was initially named as part of the Under-21s squad but has now linked up with the senior group ahead of Wednesday's friendly with Estonia.
It is the Inter defender's second call-up, having also been included for August's fixtures, while Hellas Verona midfielder Zaccagni is named in the senior squad for the first time.
Zaccagni impressed in Sunday's 2-2 draw with Milan at San Siro, with no Verona player managing more touches (57), completing more passes (23) or contesting more duels (18).
Ferrari, yet to be capped for Italy, will arrive at the Coverciano training centre on Tuesday once he and his Sassuolo team-mates have completed a period of isolation.
The Azzurri are awaiting permission from the relevant health authorities over the availability of 16 of their players due to coronavirus travel restrictions.
Roberto Mancini last week tested positive for COVID-19 and will reportedly not be in the dugout against Estonia, which comes ahead of Nations League clashes with Poland and Bosnia-Herzegovina.
He named a bumper 41-man squad last week that included uncapped attacker Pietro Pellegri after two years away, while Davide Calabria and Matteo Pessina have also been called up for the first time.
Italy enter their latest batch of fixtures unbeaten in 16 successive games, their second-longest run after going 17 without a loss between June 1997 and June 1999.