Aaron Ramsdale of Sheffield United will take his place in Gareth Southgate's 26-man squad.
"The Manchester United goalkeeper will now return to his club for further assessment and rehabilitation ahead of the 2021/22 season," the FA said in a statement.
"Ramsdale was originally a member of Gareth Southgate's pre-tournament group in Middlesbrough, having played for England at this year's UEFA U-21 Euro in Slovenia in March.
"The Sheffield United player, a 2017 UEFA U-19 Euro winner, will now follow standard Covid-19 testing protocol before entering the camp."
Ramsdale, 23, who is uncapped at senior level, joins fellow goalkeepers Jordan Pickford and Sam Johnstone in the squad.