France international winger Dembele returned to training with the squad on October 11 for the first time after injuring a knee playing for France against Hungary at the European Championship in June.
He underwent surgery on June 28 in Finland.
Barcelona are third in their group but only a point behind Benfica in second.
Sergi Barjuan has also been able to recall striker Ansu Fati, who missed two games, midfielder Frenkie de Jong, who injured a thigh in the Clasico on October 24, and defender Ronald Araujo for the match in Kiev on Tuesday.
But the interim coach will be without Gerard Pique, who strained a calf in the draw with Alaves on Saturday and striker Sergio Aguero, who was diagnosed with a heart problem after the game and remains under observation.