Five-time defending champion United States reached the Rio Olympic women's basketball semi-finals with a 47th consecutive Games victory Tuesday while Australia's run of five consecutive Olympic medals ended in tears.

Diana Taurasi and Maya Moore each scored 19 points as the Americans ripped Japan 110-64, advancing to a Thursday matchup against France, which downed Canada 68-63 to book a semi-final rematch of the 2012 gold medal game.

"We know what our game plan will be -- efficient, aggressive and unselfish," Moore said. "We're looking forward to it."

Australia left an Olympics empty handed for the first time since 1992 after Serbia delivered a shock 73-71 upset. 

The reigning European champions will face Spain, which edged Turkey 64-62 on Anna Cruz's basket at the final buzzer, in the other semi-final. 

"I know we can take it one step further because I know we have it in our hearts," Serbia's Sonja Petrovic said. "We finally played like it was now or never."

An 18-4 US run around half-time lifted the Americans comfortably ahead of Japan until the end, the Asian squad lurking within 48-46 until then.

"They were making us work. They were making us uncomfortable," Taurasi said. 

"I'm proud of this team. We didn't panic. We just kept grinding it out and making it work for us."

Taurasi had six points in the key span and Moore five.

"Responding that way was good to see," said Moore. "That's exactly what we needed. I think it's just a matter of us wearing teams down."

US captain and guard Sue Bird injured her right knee in the second quarter. It will be examined Wednesday.

The Americans are 87-1 from 1996 in Olympic and world championship games. They haven't lost in the Olympics since falling to the Commonwealth of Independent States in a 1992 semi-final.

Ana Dabovic scored 24 points, Jelena Milovanovic added 17 and Petrovic had 13 points to spark the Serbians, the only team with a losing record in the medal playoffs.

"When you look at the quality in the roster or the names on the paper, they have the better team," Petrovic said. 

"When you look at the energy and heart, that's where we were able to win out."

It was a gut-wrenching end for the Aussies, who went unbeaten in the group stage and had taken silver medals in 2000, 2004 and 2008 and bronze in 1996 and 2012.

"It's disappointing," Aussie coach Brendan Joyce said. 

"We were confident we would get through to the medal rounds so being knocked out is really difficult to swallow."

The Serbs were dancing in celebration at midcourt after the final horn.

"This was the game of our lives," Milovanovic said.

"We showed we're not European champions by accident," Dabovic said.