Marcelo will miss Real Madrid's trip to Real Betis after a back injury stopped him from travelling for Saturday's LaLiga fixture.
The Brazil international was initially named in Zinedine Zidane's 22-man squad for the game but was forced to withdraw at the last minute.
In a short statement on the club's website, Madrid revealed Marcelo would not be involved after "feeling discomfort in his back", meaning he has remained in the Spanish capital.
The 32-year-old defender was an unused substitute for the reigning champions' opening outing of the new campaign, watching on from the bench as Zidane's side drew 0-0 at Real Sociedad.
Having played the full game at the Reale Arena last weekend, Ferland Mendy seems set to continue on the left side of Madrid's defence against Betis.
As well as Marcelo, Madrid are also missing Marco Asensio and Eden Hazard. However, Isco and Lucas Vazquez could feature in Seville after recovering from injuries.