Alvaro Morata and Koke have been recalled to the Spain squad, while Marcos Llorente has earned a first senior call-up.
La Roja have been short of in-form central strikers in recent months but Morata has enjoyed a strong start to the season with Juventus, scoring six goals in seven appearances in all competitions, including three in his past two games.
Morata's last appearance for Spain was in a 5-0 Euro 2020 qualifying win over Romania in November 2019.
Atletico Madrid midfielder Koke has also returned, having last played for his country in a 4-1 friendly defeat of Wales more than two years ago.
Team-mate Marcos Llorente has earned his first call-up, while Liverpool's Thiago Alcantara is out due to problems with a knee injury and Arsenal's Dani Ceballos has been omitted.
With Diego Llorente injured, Inigo Martinez returns, as does Fabian Ruiz. Rodrigo Moreno is left out after testing positive for coronavirus this week.
Chelsea goalkeeper Kepa Arrizabalaga keeps his place despite having lost his starting spot under Frank Lampard to new signing Edouard Mendy.
Spain face the Netherlands in a friendly on Wednesday, before Nations League clashes with Switzerland and Germany.
Kepa Arrizabalaga (Chelsea), David de Gea (Manchester United), Unai Simon (Athletic Bilbao); Sergi Roberto (Barcelona), Jesus Navas (Sevilla), Sergio Ramos (Real Madrid), Eric Garcia (Manchester City), Pau Torres (Villarreal), Inigo Martinez (Athletic Bilbao), Jose Gaya (Valencia), Sergio Reguilon (Tottenham); Rodri (Manchester City), Sergio Busquets (Barcelona), Fabian Ruiz (Napoli), Sergio Canales (Real Betis), Mikel Merino (Real Sociedad), Koke (Atletico Madrid), Marcos Llorente (Atletico Madrid); Adama Traore (Wolves), Dani Olmo (RB Leipzig), Ferran Torres (Manchester City), Gerard Moreno (Villarreal), Alvaro Morata (Juventus), Ansu Fati (Barcelona), Mikel Oyarzabal (Real Sociedad).