Aaron Boupendza stepped up in the absence of Covid-stricken Pierre-Emerick Aubameyang to earn Gabon a 1-0 victory over Africa Cup of Nations debutants Comoros on Monday.
Arsenal star Aubameyang and midfielder Mario Lemina missed their team's opening Group C fixture in Yaounde after testing positive for the coronavirus last week.
Qatar-based Boupendza struck the lone goal on 16 minutes, smashing the ball high past Comoros goalkeeper Ali Ahamada from a tight angle after being played in by Louis Ameka Autchanga.
Comoros forward Moussa Djoumoi nearly equalised in the second half with a fierce long-range shot that Gabon goalkeeper Jean-Noel Amonome shovelled wide.
The Indian Ocean islanders are one of two teams appearing at the continental tournament for the first time, along with Gambia.
Only three of 11 newcomers have reached the knockout rounds since the turn of the century, although Madagascar did make it to the quarter-finals at the first attempt in 2019.
Gabon are participating for the eighth time. They have twice advanced to the last eight, in 1996 and 2012.
Patrice Neveu's side moved level on three points with Morocco, 1-0 winners over Ghana earlier in the day.
Gabon will be hoping Aubameyang can return in time to face Ghana in their second match on Friday, while Comoros have their work cut out with upcoming opponents Morocco ranked 104 places above them.