Juventus had been bidding for a fifth consecutive league and Cup double this season.

But a Duvan Zapata brace after Timothy Castagne's opener put the side from Bergamo through to a semi-final meeting with Fiorentina.

Cristiano Ronaldo's Juventus won their first trophy of the season in the Italian Super Cup this month.

But they suffered their first defeat in a domestic competition this season, as they ride high and unbeaten in Serie A.

Massimiliano Allegri's side had come from behind against Lazio in league action at the weekend with Ronaldo snatching a 2-1 win in Rome.

But the Portuguese superstar could not find a way through for a first Cup goal in Italy with Juventus also hit by defender Giorgio Chiellini going off injured in the first half-hour.

Castagne opened the scoring after 37 minutes with Zapata grabbing another two minutes later, before adding a third four minutes from time.

The Colombian ace also scored a double in a 2-2 draw when the sides last met in Serie A on December 26.

"It's only human not to always be top," said Allegri, who was banished from the touchline for dissent.

"I don't know how many games the lads have won. In Rome we had a terrible first half but we fought back. This evening everything went wrong.

"It would have been crazy to think we could win every match. It hurts to go out because we really wanted this trophy.

"Atalanta deserved their win. They were better and showed their mental strength."

Earlier in Tuscany Federico Chiesa scored a hat-trick as Fiorentina crushed a "shameful" Roma side.

Chiesa scored two early on, adding a third in the second half past overwhelmed Roma goalkeeper Robin Olsen, with Giovanni Simeone bagging a brace in the last ten minutes.

Colombian Luis Muriel and Marco Benassi also found the net against a Roma side who were reeling after throwing away a three-goal lead to draw 3-3 with Atalanta at the weekend in Serie A.

"I'm bitter, but I won't quit," said Roma coach Eusebio Di Francesco.

"I'm disappointed in the team, there are no excuses.

"I struggle to explain what happened, as it was a game we got wrong in every conceivable way. It was shameful."

Di Francesco's side had fought back after a difficult start to the season with four straight wins in Serie A and the Coppa Italia, and are now fifth in the league.

"The frustrating thing is it's so inexplicable," said the Roma boss. "Every time we think we are healed, we discover the illness is still there."
Chiesa got the first goal after seven minutes from a Cristiano Biraghi cross.

A Bryan Cristante header rattled the post before the 21-year-old Chiesa lobbed in his second over Olsen just after quarter of an hour.

Aleksandar Kolarov pulled one back for Roma after half an hour, but Fiorentina were soon two goals ahead again through Muriel.

The Colombian set up Benassi afer 66 minutes, with Simeone coming off the bench to pile more misery on Roma who finished the game with ten men after second-half substitute Edin Dzeko was sent off for a second yellow card.

"It's the hardest day of my career," said Roma sporting director Monchi.

"I've never experienced anything quite like that and we can only apologise to all the Roma fans. I can only repeat sorry, sorry, sorry."

New signing Krzysztof Piatek scored a brace on Tuesday as AC Milan beat Napoli 2-0 at the San Siro.

Five-time winners Milan will face either Inter Milan or Lazio, who play on Thursday, over two legs in the last four.