Video assistant referees have been introduced in competitions worldwide from the Premier League to the 2018 World Cup, but their decisions have often sparked controversy.
The technology will be used from the quarter-final stage of this year's Champions League, the top Asian club competition, the Asian Football Confederation (AFC) said in a statement.
The quarter-finals for West Zone teams take place on September 30 and for East Zone sides on November 25, with a single-leg final on December 5.
The AFC said the decision showed its "commitment to ensure Asian refereeing continues to set new benchmarks".
VAR was used in Asian football for the first time in the quarter-finals of the 2019 Asian Cup.
The Champions League, featuring 32 teams stretching from Saudi Arabia in the west to Japan in the East, was suspended in March due to coronavirus-related travel restrictions but is scheduled to restart next month.