The news of his death was announced by Super Lig club Konyaspor, for whom Calik had been playing this season.
Konyaspor'umuza geldiği ilk günden bu yana taraftarımızın ve şehrimizin sevgisini kazanan futbolcumuz Ahmet Çalık'ı kaybetmenin derin üzüntüsünü yaşıyoruz. Başta futbolcumuz Ahmet Çalık'ın ailesi olmak üzere hepimizin başı sağ olsun. pic.twitter.com/pmzD3SZhV6— İttifak Holding Konyaspor (@konyaspor) January 11, 2022
Calik joined Konyaspor from Galatasaray in 2020 and wore the number six shirt for the top-flight team.
Newspaper Hurriyet reported Calik's car came off the road between Ankara and Nigde.
Turkish media broadcast footage of a car said to have been the one driven by Calik, lying in a crumpled state in a field.
A statement from Konyaspor read: "We are deeply saddened by the loss of our football player Ahmet Calık, who has won the love of our fans and our city since the first day he came to Konyaspor.
"Condolences to all of us, especially to the family of our football player Ahmet Calık."
Calik's burial took place late on Tuesday after funeral prayers at Elmadag Sungur Mosque.
He had signed a three-year contract extension in November, after making a positive impact since arriving.
The Turkish Football Federation announced Calik "passed away as a result of a traffic accident".
"May Allah have mercy on the deceased; Our condolences to his family, relatives, Ittifak Holding Konyaspor Club and the Turkish football community," a statement from the federation read.
Galatasaray expressed its sorrow, saying it was news that caused "deep sadness".
Eski futbolcumuz Ahmet Çalık'ın vefat haberini derin bir üzüntüyle öğrenmiş bulunmaktayız. Merhuma Allah'tan rahmet, kederli ailesine, sevdiklerine ve Türk spor camiasına başsağlığı diliyoruz. pic.twitter.com/HscuK743Ft— Galatasaray SK (@GalatasaraySK) January 11, 2022