With the game petering out to a draw, Trujillanos midfielder Maurice Cova converted a 94th-minute penalty to lift the Venezuelan hosts to their first win in Group 1 on Wednesday (AEST).

The Strongest, which had defender Federico Pereyra sent off in the 89th minute for a second bookable offence, thought it had earned a share of the spoils after Matias Alonso's 67th-minute effort cancelled out Carlos Sosa's first-half opener.

Back-to-back defeats left The Strongest in second position, two points ahead of Sao Paulo, scheduled to play leader River Plate on Thursday (AEST), and one adrift of the Argentine giant with one match remaining.

Already assured of a place in the round of 16, Atletico Nacional preserved its 100 per cent record thanks to a 1-0 win against Sporting Cristal.

Victor Ibarbo, on loan from Cagliari having spent time at Roma and Watford, scored from the penalty spot in the 13th minute.

Ibarbo's penalty gave Nacional their fifth win from as many games without conceding a goal in Group 4.

Second-placed Huracan was also in action, playing out a goalless draw at home to winless Penarol.

Meanwhile, Group 6 leader Toluca remains unbeaten after Fernando Uribe scored twice inside six minutes late in the second half to inspire a 2-1 victory over San Lorenzo.