Despite a 2-1 defeat to Croatia on Tuesday that cost the two-time defending champions top spot in Group D, Del Bosque has kept faith with a front line spearheaded by the tournament's joint top scorer Alvaro Morata.
Italy coach Antonio Conte made seven changes from the side that lost 1-0 to Republic of Ireland on Wednesday after resting a host of his star players with top spot in Group E already assured.
Veteran goalkeeper Gianluigi Buffon returns as does Giorgio Chiellini to bolster the defence alongside his Juventus teammates Andrea Barzagli and Leonardo Bonucci.
Mattia de Sciglio stands in for the injured Antonio Candreva in the only change from Italy's opening two victories over Belgium and Sweden.
David de Gea; Juanfran, Gerard Pique, Sergio Ramos (capt), Jordi Alba; Cesc Fabregas, Sergio Busquets, Andres Iniesta; David Silva, Alvaro Morata, Nolito
Coach: Vicente del Bosque (ESP)
Gianluigi Buffon (capt); Andrea Barzagli, Leonardo Bonucci, Giorgio Chiellini; Alessandro Florenzi, Marco Parolo, Daniele De Rossi, Emanuele Giaccherini, Mattia de Sciglio; Graziano Pelle, Eder
Coach: Antonio Conte (ITA)
Referee: Cuneyt Cakir (TUR)