Hughes limped out of the first half of the 22-16 reverse at the Stade de France, while England has also now confirmed a knock for Lawes, with neither included in a 32-man selection.
Both forwards are set for scans on Tuesday (AEDT), with Exeter Chiefs' Don Armand called up.
Captain Dylan Hartley missed the Paris loss with a tight calf, but he is included as England look to deny Ireland a Grand Slam at Twickenham.
Sam Simmonds, on the bench against France, is not in the squad preparing for Sunday's (AEDT) match, when the host will be out to avoid a third consecutive loss.
It is a game that also affords Eddie Jones's side the chance to gain some revenge for last year's Six Nations reverse against Ireland, which denied England a second Grand Slam on the bounce.