After a 7-0 demolition of Sassuolo, Juve manager Massimiliano Allegri indicated he expected Matuidi to miss his side's UEFA Champions League tie with Tottenham.
After tests, Juve provided more details on the France international's injury.
Juve said Matuidi sustained "a mild-medium tear to the flexor muscles in his left thigh".
"The Bianconeri midfielder has already started therapy and further tests will be necessary to better ascertain the extent of his injury."
Juventus hosts Tottenham in the first leg of their Champions League last 16 tie on 14 February (AEDT), travelling to Wembley for the return match on 8 March (AEDT).
Meanwhile, Juventus confirmed the departure of Matuidi's fellow midfielder Tomas Rincon, Torino converting the player's loan into a permanent deal.
Torino will pay €6million for Rincon over three financial years, the Venezuela international having started only two Serie A games for Juve.